Abstract: Objective To establish the SPF rabbits breeding stock for our department,and to meet the needs of scientific research,production and test.Methods The rabbits were cleaned by cesarotomy,the young rabbits were reared in isolators by artificial breast feed.The young rabbits weaned were given with normal intestinal bacteria,such as bifidobacterium and Fusobacterium,then transfered them into the barrier system.Growing characteristics are compared between rabbits given with normal intestines microflora and non-intestines microflora rabbits.Results They were accorded with the standard of SPF rabbits of China,with microorganism of the rabbits examined.The body weights of the former increases significantly more than the latter.Conclusions The SPF rabbit breeding stock was established by cesarotomy in our department.

Key words: Cesarean section, Breast feed, SPF Rabbits, Intestinal microflora

摘要： 目的对普通级实验家兔进行剖腹净化建立SPF家兔种群,提高实验用兔的质量,满足科研、生产及检定工作需要。方法采用剖腹摘取子宫术,在无菌隔离器内以人工哺乳方法将仔兔培育成无菌兔,再将双岐杆菌等肠道正常菌接种到无菌兔体内,使其成为SPF兔;对SPF环境中植入肠道正常菌兔和未植菌兔离乳后生长性能进行观察比较。结果人工培育出的SPF兔,经微生物和寄生虫检测,符合国家SPF兔标准;植入肠道正常菌群兔体重与未植入肠道正常菌兔生长体重相比极显著增加。结论通过剖腹摘取子宫术,人工哺乳仔兔和植入肠道正常菌群的方法建立了SPF实验用兔种群。

关键词: 摘取子宫术, 人工哺乳, SPF兔, 植菌