Abstract: Objective To study the respiratoty system disease by acid gastroesophageal reflux,the guinea pig models of acid gastroesophageal reflux had been established.Methods\ After acid or distilled water were poured into the guinea pigs 16 days, for the pH values of mucous membrane at the lower esophageal segment,the totel number and eosinphil count in brochial alveolar lavage fluid(BALF) were determined,the pathological changes of esophageal,trachea and lung were observed in light microscope. Result\ Compared with the control. the pH values of mucous membrane at the lower esophageal segment were significantly decreased,the totel number of cell and eosinphil count in BALF of the model animal were significantly increased (P<0.01).The pathological changes of esophageal,trachea and lung were inflammation,epithelial cell hyperplasia. Conclusion\ Gastric juice refluxing into esophageal can damage esophageal,trachea mucous membrane and lung the guinea pig model of acid gastroesophageal reflux was a kind of the increased survival rate,simple and operated method,It could be sticding the relationship of respiratoty system disease and acid gastroesophageal reflux.

Key words: gastroesophageal reflux, respiratoty disease, model, guinea pig

摘要： 目的建立酸性胃食管反流性疾病(GERD)的豚鼠模型,用于研究胃食管反流性疾病并发的呼吸系统疾病。方法豚鼠持续灌酸或蒸馏水16天后,测其食管下段粘膜的pH值,计数支气管肺泡灌洗液(BALF)中的细胞总数及嗜酸性粒细胞计数,并研取食管、气管和肺组织标本进行病理学观察。结果模型组豚鼠食管下段粘膜的pH值明显降低,BALF的细胞总数和嗜酸性粒细胞(EOS)显著升高(P<0.01),病理变化主要表现为食管、气管和肺组织上皮增生,炎性细胞和嗜酸性粒细胞的浸润。结论胃内容物反流能造成豚鼠食管、气管粘膜和肺组织的损伤,可研究GERD并发的呼吸系统疾病和该病的病理生理情况,该制模方法成活率高、简单易操作。

关键词: 胃食管反流性疾病, 呼吸疾病, 模型, 豚鼠