Abstract: Objective To establish the credible examination methods of LPL(-/-) knock-out mice and to find out their breeding rule. Methods The mice were bred in different ways,including crossbreed,backcross and intercross. In order to determine the genotype of the mice by PCR,genomic DNA of LPL(-/-) knock-out mice was extracted from tails. Results LPL(-/-) knock-out mice was made successfully. The credible breeding process and examination methods were established as well.Conclusion The animal model of LPL(-/-) knock-out mice was developed.

Key words: Lipoprotein lipase, Gene, Knockout mice, Breeding

摘要： 目的　摸清脂蛋白酯酶基因敲除小鼠的繁殖规律,建立可靠的检测方法。方法　采用杂交、回交、互交的方法进行培育;采用从LPL(- / - )基因敲除小鼠的个体组织(鼠尾)中提取基因组DNA进行PCR分析,对其LPL(- / - )基因型作出鉴定。结果　繁育出脂蛋白酯酶基因敲除小鼠新品系,并建立了可靠的繁殖方法和检测方法。结论　建立了脂蛋白酯酶基因敲除小鼠动物模型。

关键词: 脂蛋白酯酶, 基因, 敲除小鼠, 培育