Abstract: Objective To establish a successful xenotransplantation model of a human small cell lung cancer cell line (NCI_H446) in nude mice. Methods NCI_H446 cells were transplanted subcutaneously into nude mice.After the first generation,the xenografls were transplanted subcutaneously into nude mice with serial passages for three generations.The growth features,histology,ultramicroscope structure and karyology of the successfully transplanted tumors were observed. Results After four generations of transplantation the latent period was shorten, and the spontaneous regression was not observed.The growth curve of the tumor is like “S”.The karyotype of tumor cells belongs to human karyofype.

Key words: Human, Small cell lung cancer, Nude mice, Xenotransplantation

摘要： 目的　建立成瘤稳定、潜伏期短的人小细胞肺癌NCI_H4 4 6细胞裸鼠移植瘤模型。方法　初代移植后 ,通过皮下埋块法进行裸鼠体内连续传代移植 ,并对移植瘤的生长、组织形态和超微结构、染色体核型进行观察和检测。结果　通过连续传代移植建立了成瘤稳定、潜伏期短的人小细胞肺癌NCI_H4 4 6细胞裸鼠移植瘤模型。移植瘤的组织形态和超微结构特点符合NCI_H4 4 6细胞特点 ,染色体核型属人类染色体

关键词: 人, 小细胞肺癌, 裸鼠, 异种移植