Abstract: The expression study was carried out by using molecular hybridization technique to observe the transcription levels of HSP70 mRNA in cortices, striatums and hippocampi of rats with middle cerebral artery obliterated(MCAO). HSP70 mRNA in the cortices and striatums of the model group was found remarkably expressed at 1h, 3h, 6h, 24h and 48h after MCAO, but in the hippcampi only at 24h and 48h. The findings showed that HSP70 mRNA expression is significantly related to cerebral infarction.

Key words: Molecular biology, Cerebral infarction, HSP70

摘要： 采用分子杂交技术观察了实验性脑梗塞大鼠缺血区皮质、纹状体、海马的HSP70mRNA转录水平的动态变化 ,从基因水平探讨了急性脑缺血发生发展的病理规律及机制。结果提示 :皮质、纹状体HSP70mRNA在大脑中动脉梗塞后 1h、3h、6h、2 4h和 4 8h显著表达 ,海马仅 2 4h和 4 8h显著表达 ,表明HSP70mRNA表达与脑梗塞关系非常密切。如果能针对性改变这些基因的表达 ,以提高机体对损伤的耐受能力 ,那么对脑缺血损伤的修复及预后无疑具有重要的意义

关键词: 分子生物学, 脑梗塞, 热休克蛋白基因