Abstract: Objective Kunming(KM) mouse is a kind of laboratory mice usually for biological research in China. To standardize KM colonies, biochemical markers were studied. Methods 40 KM mice were provided by Beijing institute of biological products (Beijing), Changchun institute of biological products (Changchun), North China pharmaceutical factory (Huabei), institute of laboratory animal of Chinese Medical Science Academy (CAMS) and NRLAC in KM subcolonies, respectively. 13 loci were determined by biochemical isoenzyme electrophoresis. Results There was only b type on the allelic loci of AKP1、Es1 and Trf in five KM subcolonies. In Beijing, Changchun and Huabei of KM subcolonies, Ce2 loci is a type. On the allelic loci of Es3, there were not a type, ac type and ab type in KM colonies but without Beijing and Changchun subcolonies. In CAMS colonies, there were single type of Hbb s and Idh1 a . Allelic distributing of five KM colonies was in a state of balance at some loci. The result of Genetic distance was related to the origin of colonies. Conclusions This result suggest that there are difference in KM subcolonies, standardization is require.

Key words: KM mice, Biochemical marker, Allelic frequency

摘要： 目的　为了进行昆明小鼠标准化研究 ,探讨了不同种群遗传生化位点的差异。方法　随机选取北京生物制品研究所、长春生物制品研究所、华北药厂、中国医学科学院实验动物研究所和中国药品生物制品检定所昆明小鼠各 4 0只 (雌雄各半 ) ,测定Es3等十三个生化位点的基因型分布。结果　各群在AKP1、Es1和Trf位点均为一个表现型 ,即b型 ;北京生物制品研究所、长春生物制品研究所和华北药厂的Ce2为单一的a型 ;Es3位点除北京生物制品研究所和长春生物制品研究所外 ,均无a型、ac和ab型 ;中国医学科学院实验动物研究所种群还在Hbb(s)和Idh1(a)位点只有一种表现型。各封闭群的基因频率在多数位点上处于平衡状态 ,遗传距离计算的结果表明 ,基因分布与种群的来源有密切关系。结论　不同昆明小鼠种群之间存在差异 ,需要进一步标准化

关键词: 昆明小鼠, 生化位点, 基因频率, 遗传距离