Abstract: Briefly introducing concepts and the primary technical procedure of gene targeting, and systematically introducing selective methods and gene targeting strategies. Gene targeting has been widely utilized in the fields of gene function research, construction of the pathological animal models and gene therapy.

Key words: Homologous recombination, Geng targeting, Embryonic stem cell, Animal model

摘要： 简要阐述了基因打靶的概念和基本环节 ,系统介绍了筛选方法和基因打靶的策略。基因打靶在基因功能研究、人类疾病动物模型的建立和基因治疗等方面有着广阔的应用前景

关键词: 同源重组, 基因打靶, 胚胎干细胞