Abstract: Objective:To purify bacterium protein made up of many compositions whose molecular weights are little difference.Methods:results of using sediment and layer-division are not satisfying.So I choose SDS-RAGE.In the course of electrophoresis I improve the conditions of making-gel\,electrophoresis\,dye\,decolor,and try to separate the useful composition from gel not dyed.Results:We succeed to get the composition(MW:46kD—50kD)from bacterium protein (MW:30kD—200kD).Conclusion:SDS—PAGE is a better method to purify protein.

Key words: Bacterium protein, SDS-PAGE

摘要： 对于含有多种成分且各蛋白质成分的分子量相差较小的菌体蛋白质的提纯 ,用一般的沉淀法、层析法均达不到理想效果。作者选用SDS 聚丙烯酰胺凝胶电泳的方法进行提纯 ,并尝试电泳后不经过染色 ,直接从凝胶中分离所需要的蛋白质成分。该实验对制胶、电脉、染色、脱色等各个环节进行了适当的改进 ,取得了较好的提纯效果。

关键词: 菌体蛋白质, SDS-聚丙烯酰胺凝胶电泳