Abstract: Preliminary quality comparison between 2 brand (Tiantan and Difco) artificial media for mycoplasma pulmonis has been conducted. No significant quality difference has been observed between them. For both media, the soft-agarose culture method has been proved to grow 100ul inoculum of 10 -7 dilution of positive sample while that of broth culture has been proved to grow 100ul inoculum of 10 -9 dilution of the same sample in terms of sensitivity test. When these 2 artificial medias are used for quantifying living mycoplasma cells in a control sample, Tiantan media gives rise to 8 5×10 -8 CFU/ml while Difco gives 1×10 -9 CFU/ml. When the 2 medias are used for storing mycoplamsa under -20℃, the activity of the organism decreases 1 Log level within 2 months, but declines sharply after 6 months. The growth and development of mycoplasma pulmonis on Difco medium are superior to Tiantan media.

Key words: Mycoplasma pulmonis, Artificial media

摘要： 对常用的国产培养基与进口的培养基进行了初步的质量比较实验。结果表明 :国产培养基与进口的培养基质量两者无显著差别。定性实验中 ,半流体培养基灵敏度均达到 10 - 7稀释度 ;液体培养基灵敏度均达到 10 - 9稀释度 ;定量实验中 ,两者测定同一样品的活菌数 ,国产培养基的结果为 8 5× 10 8CFU ml;国外培养基为 1× 10 9CFU ml。两种培养基用于支原体保存时 ,在 - 2 0℃条件下 2个月下降 1个对数级 ,但 6个月以上时 ,支原体活性很快下降。肺支原体在进口培养基上的发育状态优于国产培养基。

关键词: 肺支原体