laboratory animal science

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A Comparison Study on the Result of Monkey's B Virus by Using Three Different Antigens

WEI Yi,FU Min-tai,SHI Han The Research Center of primate laboratory Animals of Guangxi TIAN Ke\|gong,YU Xiu\|ling Laboratory Animal Center in Academy of Military Medical Sciences   

  • Received:2001-06-25 Revised:2001-06-25 Online:2001-06-25 Published:2001-06-25

三种不同抗原对猴B病毒抗体检测结果的比较研究

韦毅,符明泰,石寒,田克恭,遇秀玲   

  1. 广西灵长类实验动物研究中心!南宁市530022,广西灵长类实验动物研究中心!南宁市530022,广西灵长类实验动物研究中心!南宁市530022,军事医学科学院实验动物中心!北京100071,军事医学科学院实验动物中心!北京100071

Abstract: The BV antibody of Guangxi Primate laboratory animals were detected by the antigen of HSV 1、BV and BV DIA dot.The results showed by HSV\-1 was quite different from those detected by BV,BV DIA dot.The differences between them were very significant \%(P<0.01)\%,but the difference between BV and BV DIA dot,were not significant \%(P>0.05)\%.After 2,4 and 6\|month breeding,we used the same antigen to exam those monkeys with negative BV antibody detected by HSV\-1 and BV,BV DIA dot.The results showed that the turning positive rate of BV antibody detected by HSV\-1 was quite different from those of the two others \%(P<0.01)\%.Since the mid of 1999,we have exported 1389 BV negative monkeys detected by BV antigen to USA,Holland,Japan,and German soon,and all of them meet the requirements of the guests.

Key words: Rhesus monkey, B virus, HSV\-1, Antibody detecting

摘要: 采用HSV1 抗原片、BV抗原片以及BVDIAdot试剂盒对我区灵长类实验动物进行BV抗体检查。结果表明 :用HSV1 抗原片检测为阳性猴的阳性率与BV抗原片以及BVDIAdot检测为阳性猴的阳性率之间差异极显著 (P <0 .0 1) ;后两者之间阳性率差异不显著。用HSV1 抗原片、BV抗原片以及BVDIAdot检测为阴性的猴 ,分别饲养 2、4、6个月后 ,用同一种抗原复检 ,结果HSV1 检测为阴性猴的阳转率与后两者的阳转率之间的差异极显著 (P <0 .0 1)。自 1999年 8月至今 ,经BV抗原片检测为阴性的猴出口至美国、荷兰、日本、德国等国家累计 1389只 ,均符合客户要求。

关键词: 猕猴, B病毒, 单纯疱疹病毒I型, 抗体检测