Abstract: ? The nephrotic syndromed rat caused by the level of adriamycin is a good animal model and similar to the symptom of human minimal change in nephropathy. Here we report a new method causing experimental nephrotoxicity in rats by twice intravenous injection of adriamycin. Proteinuria appeared in one week after treatment. By the end of 5th. Week, proteinuria had reached the level of(512±98.9) mg/24h, and the plasma protein concentration was reduced significantly. Widespread fusion of foot processes could be found in rat glomerulus by electron microscopy. The ALB mRNA was detected by Northern and Dot blot. Albumin gene transcription was enhanced in liver of nephrotic rats and about as twice as that of the control one.

Key words: Adriamycin\ Nephropathy\ Gene transcription

摘要： 对阿霉素急性肾病模型大鼠的制作方法进行了改进，并对其生化、病理及基因转录水平进行研究。结果表明，改造后的模型血浆蛋白降低，尿蛋白升高至５１２ ±９８ ．９ ｍｇ／２４ｈ ，模型典型而稳定，成活率明显提高，达９０ ％ 。电镜观察结果符合微小病变型肾病的病理变化。鼠肝白蛋白ｍ ＲＮＡ 转录水平为对照组的２－０１ 倍。提示该模型的改造是成功的

关键词: 微小病变型肾病, 阿霉素肾病模型, 基因转录