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LI Qin ;Liu Yulin ;Wang Yuqing ;Yang Guizhong ;Lou Qinglin ;et al
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李秦,朱德生,刘玉林,王玉清,杨贵忠,娄清林
Abstract: The purpose of this study was to find out the effect of superovulation on hormone of PMSG 、 APMSG 、 hCG 、 LHRH to Rat 、 Mice、Guinea 、 Pig、 Rabbit、Goat 、 Swine and Cattle. The results indicated that 5~10 IU PMSG and hCG can make the mice's superovulation stably. and can be collected fertilized ova 40 ± 10. 40IU PMSG and HCG can make rat the same result and can be collected fertilixed ova 50±10. In rabbit 200 IU PMSG and hCG was strongly effective, 35 ±5 fertilized ova can be collected; In Goat and Swine, 1500 IU PMSG and 1000 IU APMSG or hCG was effective, 23 ±3 Fertilized ova can be collected. In cattle, 3000 IU PMSG and 1500 IU APMSG made the result, but just collected 8 ± 2 fertilized ova, In guinea pig,we can't find a proper way to surperovulate, The results suggested that PMSG and hCG are strongly valid to superovulation.
Key words: mammalia hormone, superovulation
摘要: 本文就PMSG、APMSG、hCG、LHRH等激素对大鼠、小鼠、豚鼠、家兔、绵羊、猪、牛等动物的超数排卵作用进行了系统探索,结果发现PMSGhCG各5~10IU可致小鼠产生稳定的超排、取卵数可达50±10个;用200IU加等量hCG可使大鼠稳定超排,取卵数可达50±10个;用400IUPMSG和等量hCG可使家兔产生稳定起排、取卵数可达35±5个;使用PMSG1500IU和APMSG100IU可使羊产生超排、取卵数可达25±5个:在猪使用1500IUPMSGT和1000IUhCG平均获得卵26±5个;,使用PMSG3000IU和1500IUAPMSG,平均可稳定获得8±12个卵,而在豚鼠得不到稳定起排卵的结果。所有这些结果提示我们PMSG几乎对各种哺乳动物的超排作用显著,且有稳定的剂量可寻,而hCG的促超排也是很显著的。
关键词: 哺乳动物, 激素, 超数排卵
LI Qin ;Liu Yulin ;Wang Yuqing ;Yang Guizhong ;Lou Qinglin ;et al. Research for the Effect of Superovulation on Kinds of Hormone to Mammalia[J]. laboratory animal science.
李秦,朱德生,刘玉林,王玉清,杨贵忠,娄清林. 不同激素对哺乳动物超数排卵作用的研究[J]. 实验动物科学.
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