Abstract: The blood circulatory system of nude mice has been used to study the anti-experimental metastasis effects of LAK cells activated in vitro with interleukin-2 on the highly metastatic tumor lines moving in blood. On the first day of the test, 3×10~7 LAK cells and 1×10~6 tumor cells were mixed and injected tailveinly to each of the mice in test group (n=5), at the effectors/targets ratio of 30:1; while in the controlled group, each mouse was only injected tailveinly with 1×11~8 tumor cells. All of the mice were injected (ip) with rIL-2 300 cu/gram body weight dayly for seven days. As a result, none of the mice in test group was seen with tumor growth (0/5), eight weeks after injection; and all of the mice in controlled group were fount out with metastatic tumor formation in lungs, lymphonodes, rachis and subskin (5/5). The results indicated that LAK cells could not only exhibit the antitumor effecs in vitro, but also catch and kill metastatic cancer cells in the blood circulation.

Key words: interleukin-2(IL-2), LAK celIs, nude mice, tumor metastasis

摘要： 利用裸鼠循环系统为实验体系,建立高转移性癌细胞实验转移模型。在此基础上,将体外经白细胞介素2活化的人LAK细胞与高转移性癌细胞同时注射于裸鼠尾静脉内;将未经活化的淋巴细胞与癌细胞一同注射,作为对照,观察LAK细胞抗实验性转移作用。结果表明,每只接受3×10~7 个LAK细胞与1×10~6 个肿瘤细胞的实验组5只小鼠,注射细胞后全部健康存活,无一生长肿瘤。而接受与实验组相同数量的淋巴细胞与肿瘤细胞的对照组5只小鼠,分别于注射后第五周在皮下、脊柱旁及肺脏内出现转移瘤生长。说明LAK细胞不仅在体外具有明显的抗肿瘤作用,在裸鼠内也能够抑制高转移癌系实验性转移的发生。

关键词: LAK细胞, 裸鼠, 抗转移