Objective To improve the model’ s success rate and the reliability of the experimental result,

the existing modeling method and evaluation indexes were optimized, and a comparative evaluation of two

different strains of mouse-specific dermatitis models, BALB / c and C57BL / 6J, was performed. Method

At the age of 8 - 10 weeks, BALB / c and C57BL / 6J mice were divided into control and model groups

( n = 8) . 2, 4-dinitrochlorobenzene (DNCB) was used to induce specific dermatitis (AD) -like changes

in mice. The incidence degree of dermatitis, the number of scratching and body weight of mice were

dynamically observed and counted. The spleen index, peripheral blood immune cells, and serum levels of

inflammatory factors were collected from control and model mice at the end of the experiment for statistical

analysis. Result On the third day of the experiment, mice from both strains developed skin swelling,

erythema, and other dermatitis phenotypes, which worsened as the experiment progressed.

When compared to control mice of the same strain, the mice in each model group had significant dermatitis, as 

atopic dermatitis, improved protocol, C57BL / 6J mice, BALB / c mice

目的 对现有的建模方法和评价指标进行优化以提高模型的成功率和实验结果的可靠性,同时,对 BALB / c

和 C57BL / 6J 两种不同品系的小鼠特异性皮炎模型进行了对比评价。 方法 8 ~ 10 周龄 BALB / c 和 C57BL / 6J 小鼠

各 16 只,分为对照组和模型组(每组 8 只) ,使用 2,4-二硝基氯苯( DNCB) 诱导小鼠产生特异性皮炎( AD) 样改变,

动态观察并统计小鼠皮炎发病程度,抓挠次数和体质量等,在实验观察的终点收集对照组和模型组小鼠脾指数,对

外周血免疫细胞变化和血清相关炎症因子水平等相关数据进行统计分析。 结果 实验第 3 天,两种品系模型组小

鼠均开始出现皮肤肿胀、红斑等皮炎表型,随时间进展皮炎症状进一步加重。 与同品系对照组小鼠相比,各模型组

小鼠有明显的皮炎表现,脾指数、抓挠次数、外周血免疫细胞、相关炎症因子水平均高于其对照组水平。 BALB / c 小

鼠的皮炎表型更易观察,但各指标与 C57BL / 6J 小鼠有较大差异。 结论 两种品系小鼠都可用于 AD 小鼠模型构

建。 与 C57BL / 6J 小鼠相比,BALB / c 小鼠的皮炎症状更为明显,皮炎恢复能力弱。

特异性皮炎, 造模方法改良, C57BL / 6J 小鼠, BALB / c 小鼠