Objective To evaluate the applicability of molecular pathogen detection and serological testing method in the detection of pneumonia virus of mice ( PVM) . Method BALB / c and C57BL / 6J mice, half male and half female, aged 3 weeks, were infected with PVM via nasal droplets. Oro pharyngeal swabs and tail tip blood were collected from each experimental mouse at 1 week, 2 weeks, 3 weeks, 1 month, 2 months, and 3 months post-infection, and were tested using PCR and ELISA method. Result One week after infection with PVM, the detection rate of PVM nucleic acid in oropharyngeal

swabs of mice from both strains was 70% to 100%, which decreased and had higher CT values in the

following 2 weeks and 3 weeks, with no detection after 1 month; peripheral blood PVM antibodies were

not detected in the first week, but the detection rate was 80% to 100% in the following 2 weeks and 3

weeks, with all being detected from 1 to 3 months, and the optical density of PVM antibodies detected by

ELISA continued to increase, maintaining a high antibody level in the 2nd and 3rd months. Conclusion

PCR detection of oropharyngeal swab samples combined with serological testing technology can be applied

to the quality control of PVM in mice.

PVM, oral swab, grows and decline regularity

目的 评价分子病原学及血清学检测方法在小鼠肺炎病毒( PVM) 检测中的适用性。 方法 3 周龄 BALB / c

和 C57BL / 6J 小鼠雌雄各半,滴鼻感染 PVM,在感染后 1、2、3 周,1、2、3 月采集各实验鼠口咽拭子及尾尖血,分别应

用 PCR 和 ELISA 方法进行检测。 结果 两品系小鼠感染 PVM 病毒 1 周后,口咽拭子 PVM 核酸检出率为 70% ~

100%,随后 2 周和 3 周检出率降低,CT 值增高,1 个月后无检出;外周血 PVM 抗体 1 周无检出,随后 2、3 周检出率

为 80% ~ 100%,1 至 3 月全部检出,且 PVM 抗体 ELISA 检测吸光度持续增高,2 月和 3 月仍维持较高的抗体水平。

结论 小鼠口咽拭子样品的 PCR 检测结合血清学检测技术,可应用于小鼠 PVM 的质量控制。

小鼠肺炎病毒, 口咽拭子, 消长规律