Abstract: Objective　To investigate the effect of Shenqi Shiyiwei Granules (SQS) on cancer-related fatigue (CRF) in CT26 colorectal cancer mice.Methods　Thirty BALB/c mice were randomly divided into a blank group, model group, and SQS group [1.56 g/(kg·d) ],10 mice per group. Except for the blank group, the other two groups were subcutaneously inoculated with CT26 cells in the right hind limb to establish a tumor model. After successful modeling, the treatment group received SQS by gavage for three weeks. Body weight and tumor volume were monitored weekly. Exercise endurance and spontaneous activity were assessed using the forced swimming test and open field test. Liver and gastrocnemius glycogen content, serum urea nitrogen (BUN), lactate (LA) levels, and lactate dehydrogenase (LDH) activity were measured by colorimetric assays. Complete blood counts were analyzed by using an automated hematology analyzer. Spleen and tumor weights were recorded. Pathological changes in gastrocnemius muscle and epididymal adipose tissue were observed by HE staining.Results　Compared with the model group, mice in the SQS group showed significantly increased body weight, prolonged load bearing swimming time, and increased total distance traveled in the open field test, while tumor volume and weight were significantly reduced, the differences were statistically significant (P<0.05). Liver and gastrocnemius glycogen levels were higher, while serum BUN, LA, and LDH were significantly lower (P<0.05). Blood parameters, including red blood cells, hemoglobin, platelet count and white blood cells, were significantly improved, and the differences were statistically significant (P<0.05). Histopathological analysis revealed attenuated gastrocnemius muscle atrophy and enlarged epididymal adipocytes in the SQS group. Conclusion　SQS alleviates CRF by improving energy metabolism, reducing tissue damage, and regulating hematological parameters.

Key words: cancer-related fatigue, CT26 colorectal cancer, Shenqi Shiyiwei Granules

摘要： 目的　探讨参芪十一味颗粒(SQS)对CT26结直肠癌荷瘤小鼠癌因性疲乏(CRF)的效果。方法　将30只 BALB/c小鼠随机分为空白组、模型组和SQS组[1.56 g/(kg·d)],每组10只。除空白组外,其余两组小鼠于右后 肢皮下接种CT26细胞建立肿瘤模型。造模成功后SQS组连续灌胃给药3周。每周监测各组小鼠体质量和肿瘤体 积变化;通过负重游泳实验和旷场实验评估小鼠运动耐力及自主活动能力;采用比色法检测小鼠肝组织和腓肠肌 糖原含量,血清尿素氮(BUN)、乳酸(LA)水平和乳酸脱氢酶(LDH)活性;采用全自动血细胞分析仪检测血常规;称 量脾和肿瘤重量;使用HE染色观察腓肠肌和附睾脂肪组织病理形态学改变。结果　与模型组相比,SQS组小鼠体 质量显著增加,负重游泳时间延长,旷场活动总距离增加,肿瘤体积和质量明显降低,差异均有统计学意义(P< 0.05);肝组织和腓肠肌糖原储备增加,血清BUN、LA水平及LDH活性显著降低,差异均有统计学意义(P<0.05); 红细胞、血红蛋白、血小板计数和白细胞等血液指标明显改善,差异均有统计学意义(P<0.05);组织病理学结果显 示,SQS组小鼠腓肠肌萎缩程度减轻,附睾脂肪细胞直径增大。结论　SQS可通过改善能量代谢、减轻组织损伤和 调节血液指标等多种途径发挥缓解CRF的作用。

关键词: 癌因性疲乏, CT26结直肠癌, 参芪十一味颗粒