Abstract: Objective　To clarify the biological characteristics of commercialized immunodeficient mice in China and provide a scientific basis for their standardized management and quality control.Methods Fourteen strains of immunodeficient mice from seven domestic enterprises were selected, which were classified into three categories, T cell-deficient, T and B cell-deficient, and T, B and NK cell-deficient. Their organ coefficients, hematological indicators, peripheral blood lymphocyte subsets, and histological characteristics of immune organs were detected and compared with those of age-matched BALB/c mice. Results　All three types of immunodeficient mice exhibited phenotypic characteristics corresponding to the deficiency of target immune cells. The thymus coefficients were significantly lower than those of control group, lymphocyte-related indicators (LYMPH, LYMPH%, CD3+ T cells, etc.) were significantly downregulated, and indicators such as neutrophil percentage (NEUT%) were significantly upregulated. However, statistical differences were observed in some hematological and lymphocyte subset indicators among mice of the same strain from different enterprises (P<0.05, P<0.01).Conclusion The overall quality of commercialized immunodeficient mice in China is stable, and their biological characteristics are basically consistent with those of immunodeficient mice. However, significant variations in biological indicators within certain strains deviate greatly from the average, indicating that standardization needs to be further improved.

Key words: immunodeficient mice, hematology, lymphocyte subsets, organ coefficients

摘要： 目的　明确我国商品化免疫缺陷小鼠的生物学特征,为其标准化管理及质量控制提供科学依据。方法　选 取国内7家企业的14个品系免疫缺陷小鼠(T细胞缺陷,T、B 细胞缺陷,T、B、NK 细胞缺陷),检测其脏器系数、血 液学指标、外周血淋巴细胞亚群及免疫器官组织学特征,并与同周龄BALB/c小鼠对比。结果　3类免疫缺陷小鼠 均呈现对应免疫细胞缺失的表型特征。胸腺系数显著低于对照组,淋巴细胞相关指标淋巴细胞(LYMPH)、淋巴细 胞百分率(LYMPH%)、CD3+T细胞等显著下调,中性粒细胞百分率(NEUT%)等指标显著上调;部分血液学及淋巴 细胞亚群指标在不同企业同品系小鼠间差异有统计学意义(P<0.05,P<0.01)。结论　我国商品化免疫缺陷小鼠 大部分质量稳定,生物学特性基本符合免疫缺陷小鼠特征,但个别品系内的生物学指标与平均水平有较大差异,尚 需进一步提升标准化水平。

关键词: 免疫缺陷小鼠, 血液学, 淋巴细胞亚群, 脏器系数