Phenotypical Difference and Genetic Quality Control in C57BL/6 Substrains

doi:10.3969/ j. issn.1006-6179.2026.02.018

Laboratory Animal Science ›› 2026, Vol. 43 ›› Issue (2): 100-104.DOI: 10.3969/ j. issn.1006-6179.2026.02.018

(1.Shanghai Laboratory Animal Research Center, Shanghai 201203, China) (2. Shanghai BK/KY Biotechnology Co.,Ltd., Shanghai 201203, China)

Received:2024-08-07 Online:2026-02-28 Published:2026-05-05

C57BL/6亚系小鼠的表型差异及遗传质量控制

(1.上海实验动物研究中心,上海　201203) (2.上海必凯科翼生物科技有限公司, 上海　201203)

通讯作者: 赵　莹(1982—),女,博士,副研究员,研究方向为实验动物种群遗传质控研究及遗传学研究。E-mail:zhaoying@slarc.org.cn。
作者简介:赵丽亚(1981—),女,硕士,工程师,研究方向为实验动物种群遗传控制研究。E-mail:zhaoliya3002@163.com。

Abstract

Abstract: The C57BL/6 mouse is the most widely used experimental rodent to date. C57BL/6J and C57BL/6N represent the major lineages of C57BL/6 mouse, from which a large number of substrains have been derived following long-term independent breeding. Genetic background differences among substrains are likely to cause phenotypic divergence, which directly affects the reliability and reproducibility of experimental result. If C57BL/6 substrains with unclear genetic backgrounds are used in experiments, potential genetic differences may cause unpredictable interference to the research result. Therefore, institutions responsible for breeding C57BL/6 mouse colonies should prioritize and implement genetic quality control. Scientific measures should be taken to promptly identify genetic contamination or substrain admixture, maintain the stable and well-defined genetic background of the substrains, and eliminate interference with research caused by mixed genetic backgrounds at the source.

Key words: C57BL/6 mouse, substrain, genetic differences, phenotypic differences, genetic quality control

摘要： C57BL/6小鼠是迄今为止应用最广泛的实验室啮齿类动物,其中C57BL/6J和C57BL/6N为两大核心谱系, 经长期繁育又各自衍生出多个亚系,亚系间的遗传背景差异易引发表型分化,进而直接影响实验结果的可靠性。若选用遗传背景不明确的C57BL/6亚系开展实验,其潜在的遗传差异可能对研究结果产生不可预估的干扰。因此,C57BL/6种群的繁育机构需重视并落实其遗传质控工作,通过科学措施及时发现遗传污染或亚系混杂问题,维持亚系的遗传背景稳定与清晰,从源头杜绝遗传背景混杂对研究的干扰。

关键词: C57BL/6小鼠, 亚系, 遗传差异, 表型差异, 遗传质控

CLC Number:

Q95-3

Zhao Liya, Shen Miao, Jin Yichen, Zhao Ying. Phenotypical Difference and Genetic Quality Control in C57BL/6 Substrains[J]. Laboratory Animal Science, 2026, 43(2): 100-104.

赵丽亚, 沈　淼, 金奕宸, 赵　莹. C57BL/6亚系小鼠的表型差异及遗传质量控制[J]. 实验动物科学, 2026, 43(2): 100-104.

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