Abstract: Objective To investigate the effective time and sensitivity of sentinel mice in monitoring mite and flagellate infection in IVC System in barrier environment under different types of soiled bedding ( corncob and shavings) , and to explore the actual monitoring ability of sentinel mice. Method 42 sentinel mice ( 4-week-old female ICR) were randomly divided into 6 groups. The sentinel mice were raised with 100% of the feeding soiled bedding ( corncob or shavings) from mite and flagellate double positive mice, or directly placed in the cage of the mice infected with both mites and flagellates. Then the sentinel mice were detected at different times after exposure. Result After 6 weeks, the mite infection rates of the sentinel mice added soiled corncob and shavings bedding were 20% and 60%, respectively, but the detection of flagellate in both groups was negative. After 8 weeks, the mite infection rate of sentinel mice with 100% soiled corncob bedding was 33. 33%, and the flagellate infection rate was 16. 67%; the mite infection rate of sentinel mice with 100% soiled shaving bedding was 83. 33% and no flagellate was detected. The sentinel mice, which were directly raised with mite and flagellate double infected mice in the same cage, were 100% infected with mites and flagellates on day 25. Conclusion The corncob soiled bedding is less sensitive than shavings soiled bedding in monitoring mite infection, suggesting that shavings soiled bedding exposure method has reference significance for mice whose population is not easily expanded.

Key words: sentinel mice, barrier environment, IVC system, parasitic infection

摘要： 目的 验证屏障环境中的 IVC 系统内不同类型(玉米芯和刨花)脏垫料条件下的哨兵小鼠监测螨虫和鞭毛虫 感染的有效时间和敏感度,以探讨其实际监测能力。 方法 将 42 只哨兵小鼠( 4 周龄雌性 ICR 小鼠) 随机分为 6 组,间接添加螨虫和鞭毛虫双阳性小鼠的 100%饲养脏垫料(玉米芯或刨花)给哨兵小鼠使用,或直接放置哨兵小鼠 到螨虫和鞭毛虫双重感染的小鼠饲养笼内,暴露不同时间后检测哨兵小鼠的感染情况。 结果 6 周后,添加玉米芯 和刨花脏垫料的哨兵小鼠螨虫感染率分别为为 20% 和 60%,但两组哨兵鼠鞭毛虫检测均为阴性。 8 周后,添加玉 米芯和刨花脏垫料的哨兵小鼠螨虫感染率分别为 33. 33%和 83. 33%,但只有玉米芯饲养组鞭毛虫呈阳性( 感染率 为 16. 67%) 。 与螨虫和鞭毛虫双阳性小鼠共饲养在同一个笼内的哨兵小鼠在接触 25 d 时 100%感染了螨虫和鞭毛 虫。 结论 玉米芯脏垫料暴露在监控螨虫感染时不及刨花脏垫料敏感,提示刨花脏垫料暴露法对于种群不容易扩 大的鼠群有借鉴意义。

关键词: 哨兵小鼠, 屏障环境, IVC 系统, 寄生虫感染