Abstract: Objective To explore the disinfection effects of 6 disinfectants, namely 3% hydrogen peroxide, 75% ethanol, 84 disinfectant, brand 1 disinfectant, brand 2 disinfectant and potassium bisulfate, in animal houses in barrier environment, so as to provide reference for actual disinfection work.  Method The 6 disinfectants were diluted accordingly according to the product instructions, and the cage boxes of SPF mice were prepared, and the dirty bedding was fully rubbed to make the microorganisms fully contact with the gloves, and the experimental group used sterile gloves to rub the dirty bedding and then disinfect it with different method, Then sampled the surface of the gloves, the sampling positions were all palms and backs, the sampling area was 5 cm×5 cm, the sample solution was smeared on the agar petri dish for bacterial culture, Then positive control was directly sampled and cultured after rubbing the dirty bedding. The negative control samples were smeared on the surface of sterile medical gloves, All experimental groups and the positive and negative control groups were treated with 3 sterile agar medium for 48 h at 37 ℃ to count the growth colonies, and the disinfection effects of different treatment method were compared. Result No colony growth was observed after 75% ethanol disinfection. Brand 2 disinfectant solution was diluted and soaked in 1 ∶ 200 and 1 ∶ 400 for disinfection, and no colony growth was observed in the sampling culture. The average number of colonies grown after 3% hydrogen peroxide disinfection was ( 0. 33 ± 0. 47) cfu / dish. The average number of colonies after disinfection of brand 2 disinfectant solution (1 ∶ 128 dilution) was (53. 3±3. 9) cfu / dish, the average number of colonies after spray disinfection of brand 1 disinfectant solution ( 1 ∶ 100 dilution ) was ( 52. 3± 4. 5 ) cfu / dish, potassium persulfate ( 1 ∶ 100 dilution) was ( 68. 3 ± 5. 3) cfu / dish, 84 disinfectant solution ( 1 ∶ 50 dilution) average colonies were (62. 0±2. 4) cfu / dish, and potassium persulfate (1 ∶ 200 dilution) and other disinfectant spray disinfection method, the average number of colonies was (145. 7±5. 7) cfu / dish, and the average number of colonies after spraying of brand 2 disinfectant (1 ∶ 200 dilution) was (178. 3 ±2. 1) cfu / dish. The number of colonies grew in the positive control > 500 cfu / dish, and the colonies grew in the negative control. Conclusion The order of disinfection effect is 75% ethanol, 3% hydrogen peroxide spray and brand 2 disinfectant soaking, high concentration ( brand 1 disinfectant, potassium persulfate and 84 disinfectant) spray disinfection, and low concentration potassium persulfate and low concentration brand 2 disinfectant spray. The validation result can provide a reference for the use of disinfectants in animal rearing processes. 

Key words: barrier-level environment, experimental animal, disinfection verification

摘要： 目的 初步探索屏障环境动物房内 3%过氧化氢、75%乙醇、84 消毒液、品牌 1 消毒液、品牌 2 消毒液和过硫 酸氢钾 6 种消毒剂的消毒效果,为实际消毒工作提供参考。 方法 参照产品说明书对此 6 种消毒剂进行相应的稀 释,准备饲养过 SPF 小鼠的笼盒,均充分揉搓其脏垫料,使微生物充分与手套接触,实验组采用无菌手套揉搓脏垫 料后用不同方法消毒,再对手套表面采样,采样位置均为手心与手背,采样面积为 5 cm×5 cm,样液涂抹于琼脂培养 皿进行细菌培养,阳性对照为揉搓脏垫料后直接采样培养,阴性对照采用无菌医用手套表面涂抹平板,所有实验组 与阴阳性对照组均使用 3 个无菌琼脂培养基 37 ℃ 培养 48 h 后对生长菌落计数,比较不同处理方法的消毒效果。 结果 75%乙醇消毒未见菌落生长。 品牌 2 消毒液 1 ∶ 200 与 1 ∶ 400 稀释浸泡消毒,采样培养未见菌落生长。 3%过氧化氢消毒后平均生长菌落数为( 0. 33±0. 47) cfu / 皿。 品牌 2 消毒液( 1 ∶ 128 稀释) 消毒后平均菌落数在 ( 53. 3±3. 9) cfu / 皿、品牌 1 消毒液( 1 ∶ 100 稀释) 喷 雾 消 毒 后 平 均 菌 落 数 ( 52. 3 ± 4. 5) cfu / 皿、过 硫 酸 氢 钾 ( 1 ∶ 100 稀释) 喷雾消毒后平均菌落数在( 68. 3±5. 3) cfu / 皿、84 消毒液( 1 ∶ 50 稀释) 平均菌落( 62. 0± 2. 4) cfu / 皿、过 硫酸氢钾( 1 ∶ 200 稀释) 等消毒液的喷雾消毒方式平均菌落数在( 145. 7± 5. 7) cfu / 皿,品牌 2 消毒液( 1 ∶ 200 稀 释) 喷雾后平均菌落数( 178. 3±2. 1) cfu / 皿。 阳性对照菌落生长数>500 cfu / 皿,阴性对照无菌落生长。 结论 消 毒效果排序依次为 75%乙醇、3%过氧化氢喷雾和品牌 2 消毒液浸泡,高浓度( 品牌 1 消毒液、过硫酸氢钾与 84 消毒液) 喷雾消毒,低浓度过硫酸氢钾与低浓度品牌 2 消毒液喷雾。 验证结果可为动物饲养过程中消毒剂的使 用提供参考。

关键词: 屏障级环境, 实验动物, 消毒验证