Abstract: Objective The traditional method of collecting arterial blood from mouse heart puncture has many problems, such as high difficulty and low success rate. An improved method is proposed under the existing conditions to ameliorate the above problems. Method Forty male Balb / c mice aged 8 weeks were randomly divided into the traditional method group and modified method group. Arterial blood collection and blood gas analysis were performed by percutaneous left ventricular puncture and left ventricular puncture through muscle layer after skin stripping, respectively. The puncture success rate, maximum blood volume and blood gas analysis of the two groups were counted, and the result were compared and analyzed. Result The puncture success rate of the traditional method was 40%, and the maximum blood collection volume was ( 0. 41 ± 0. 13) mL. The puncture success rate of the modified method was 90%, and the maximum blood collection volume was (0. 51±0. 10) mL. The result showed that the success rate and maximum blood volume of the modified method were significantly higher than those of the traditional method, and the differences were statistically significant (P<0. 01, P<0. 05) . In terms of blood gas analysis, the pH of the traditional method group was 7. 08 ± 0. 12, and that of the modified method group was 7. 09 ± 0. 13 ( t = - 0. 17, P = 0. 87) . The PCO₂ of the traditional method group was 35. 60±6. 02, and the PCO₂ of the improved method group was 32. 33±4. 09 ( t = 1. 27, P = 0. 22) . The oxygen partial pressure ( PO₂ ) of the traditional method group was 90. 13±10. 23, and that of the modified method group was 97. 13 ± 14. 76 ( t = - 1. 10, P = 0. 29) . The blood oxygen saturation ( SO₂ ) of the traditional method group was 87. 00 ± 5. 29, and that of the modified method group was 91. 63 ± 4. 41 ( t = - 1. 90, P = 0. 78 ) . The above blood gas analysis result showed no statistical difference. Conclusion The modified method of collecting arterial blood from mouse heart puncture is simple and visualized, with high success rate and good repeatability.

Key words: mouse, cardiac puncture, arterial blood, blood gas analysis

摘要： 目的 传统的小鼠心脏穿刺动脉血采集方法具有难度高、成功率低等问题而影响血气测定。 在现有条件下,为了改善上述问题提出一种改良方法。方法 将 40 只 8 周龄雄性 C57BL / 6 小鼠随机分为传统方法组和改良方法组,分别以经皮左心室穿刺和剥离皮肤后经肌肉层左心室穿刺进行动脉血采集及血气分析。 统计两组小鼠穿刺成功率、最大采血量、血气分析等,并对结果进行比对分析。 结果 传统穿刺组小鼠的心脏穿刺成功率为 40%,最大采血量为(0. 41±0. 13) mL;改良穿刺组小鼠的穿刺成功率为 90%,最大采血量为( 0. 51±0. 10) mL。 结果显示,改良方法的成功率及最大采血量均显著高于传统方法,差异具有统计学意义( P< 0. 01,P< 0. 05) 。 在血气分析方面,传统方法组的酸碱度( pH)为 7. 08±0. 12,改良方法组的 pH 为 7. 09±0. 13( t = -0. 17, P = 0. 87) ;传统方法组的二氧化碳分压( PCO2)为 35. 60±6. 02,改良方法组的 PCO₂ 为 32. 33±4. 09( t = 1. 27, P = 0. 22) ;传统方法组的氧分压( PO₂)为 90. 13±10. 23,改良方法组 的 PO₂ 为 97. 13 ± 14. 76 ( t = - 1. 10, P = 0. 29) ;传统方法组的血氧饱和度( SO₂) 为87. 00±5. 29,改良方法组的 SO₂ 为 91. 63±4. 41( t = - 1. 90, P = 0. 78) ,以上血气分析结果均未显示出统计学差异。结论 改良小鼠心脏穿刺动脉血采集方法直观简便,成功率高,重复性好。

关键词: 小鼠, 心脏穿刺, 动脉血, 血气分析