Abstract: Objective To establish a stable, simple and safe animal model and evaluation system for blast-related traumatic brain injury ( bTBI) . Method After anesthesia, rats were placed inside a steel pipe with an inner diameter of 6 cm, and explosive devices devices with a TNT content 0. 1 g were placed 7 or 9 cm away from the head of the rats. The physiological characteristics of the rats were observed before and after the explosion. The Garcia scoring method was used to score their neurological function of the rats. 6 hours later, the brain tissues were observed the changes, evaluated the cerebral edema, and observed pathological changes in brain tissues HE staining. Result After the experiment, the nerve function of the rats was damaged and the brain tissue edema appeared. HE staining was used to observe the bleeding of the brain tissue. The explosive device was 9 cm away from the head of the rats, and the brain explosion injury was more serious at 7 cm away. Conclusion It is safe, stable and a reliable method to construct the model of brain blast injury in rats with simple modeling device.

Key words: brain blast injury, animal model, rat

摘要： 目的 探讨一种具有稳定、简单、安全的大鼠脑爆震伤( bTBI)模型的建立方法及评价体系。 方法 大鼠麻醉后,放置在内径 6 cm 的钢管内,分别在距离大鼠头部 7、9 cm 位置放置 0. 1 g TNT 含量的爆炸装置。 观察爆炸前后大鼠生理特征。 采用 Garcia 评分法对大鼠进行神经功能评分,6 h 后处死大鼠,取脑组织观察变化,评价脑水肿情况,观察脑组织 HE 染色病理变化。 结果 实验后大鼠神经功能受损,脑组织出现水肿,HE 染色观察脑组织出血,爆炸装置距离大鼠头部 9 cm 位置可使大鼠产生脑爆震伤,7 cm 位置损伤较为严重。 结论 使用简易造模装置可制备大鼠脑爆震伤模型,安全、稳定、是一种可靠的造模手段。

关键词: 脑爆震伤, 动物模型, 大鼠