Abstract: Objective In order to facilitate the acupuncture and moxibustion experiment in rats, a supine position fixing device which is suitable for the acupuncture and moxibustion operation of acupoints in abdomen and lower limbs of rats was designed on the basis of following the 3R principles. Method Discarded coke bottles were collected and processed into the fixation device according to the predesigned program. By using the natural instince of rats like seeking holes,the rat were bound in supine posture bottle, then the bound rat were stably placed between two fixed bottom plates. The acupoints of the abdomen and lower limbs were fully exposed, and the rats were treated with electroacupuncture. It was compared between the device fixation group and the traditional restraint fixation group from the aspects of the number of operator, the operation time, and the different states of rat undergoing fixation and treatment. Result Compared with the restraint fixation, the fixation of the rat by this device can be completed by only one person, and the fixation time of a single rat is significantly shortened (P<0. 01) . The various conditions of the rat during the fixation and treatment are obvious improve. Conclusion The materials of this device are easy to obtain and easy to modify. Compared with other fixation methods, it has the advantages of simple operation, reliable fixation, saving manpower and time, etc. It is a more applicable method for acupuncture and moxibustion operation on the abdomen and lower limbs of rats, and it provides a new safe and reliable choice for rat fixation in the experiment and is worthy to promote.

Key words: rat, acupuncture, supine device fixation method, restraint fixation

摘要： 目的 为方便大鼠针灸类实验操作,在遵循 3R 原则的基础上,设计出一种适用于大鼠腹部及下肢腧穴针灸操作的仰卧位固定装置。 方法 本装置选取可乐瓶,按照设计方案进行裁切改制而成,利用大鼠擅于钻洞习性,使大鼠于固定瓶内以仰卧位姿势合理固定,平稳置于两固定底板之间,使腹部及下肢腧穴充分暴露,对大鼠进行电针治疗。 从操作所需人数、操作时间、大鼠接受固定及治疗中的不同状况等方面,进行装置固定法与传统束缚固定法之间的比较。 结果 与束缚固定法相比,该装置法固定大鼠仅需 1 人即可完成,单只大鼠固定时长显著缩短(P<0. 01) ,大鼠在接受固定及治疗中的各类状况均得到明显改善。 结论 本装置制作材料易获取、改装易完成。与其他固定法相比,本法具有操作简单、固定可靠、节省人力、时间等优势,是一种适用于大鼠腹部及下肢腧穴针灸操作的固定方式,为针灸类实验大鼠的固定提供一种新的安全可靠的固定方法。

关键词: 大鼠, 针灸, 仰卧位装置固定法, 束缚固定法