Abstract: Objective To observe the effects of acupuncture on bone metabolism and bone quality in ovariectomized osteoporosis model rats, and to explore the mechanism of acupuncture in treating osteoporosis in youth. Method Forty female SD rats were randomly divided into a normal group, a sham operation group, a model group and a acupuncture group, with 10 rats in each group. The model group and acupuncture group used the ovary to prepare the rat model of osteoporosis in youth. The model group did not carry out intervention treatment. The acupuncture group was given electroacupuncture to stimulate “ Shenshu” , “ Zusanli” and “Xuanzhong” at the 2nd week after operation, once a day, for 8 consecutive weeks. The contents of serum alkaline phosphatase ( ALP ) and osteocalcin ( OC) were detected by enzyme-linked immunosorbent assay ( ELISA ) ; Femoral bone mineral density ( BMD ) and bone morphological parameters were measured by micro CT. Result After 8 weeks of intervention, compared with the normal group and sham operation groups, ALP and OC increased in the model and acupuncture groups, BMD, BV / TV and Tb. Th decreases,and Tb. Sp increased (P < 0. 05) , indicating the success of osteoporosis modeling. Compared with the model group, ALP and OC in the acupuncture group decreased, BMD、 BV / TV and Tb. Th increases, and Tb. Sp decreased ( P < 0. 05 ) , indicating that acupuncture can improve bone mineral density and bone microstructure. Conclusion Acupuncture has the effect of preventing osteoporosis in youth, and its mechanism may be related to the promotion of bone formation and repair, the increase of bone density, and the enhancement of the structural strength of bone trabeculae.

Key words: acupuncture, osteoporosis, youth rats, micro-CT

摘要： 目的 观察针刺对青年期去卵巢骨质疏松模型大鼠骨代谢和骨质量的影响,探讨其治疗青年期骨质疏松症的作用机制。 方法 将 40 只 SD 雌性大鼠随机分为正常组、假手术组、模型组和针刺组,每组各 10 只。 模型组和针刺组通过去卵巢制备青年期骨质疏松症大鼠模型,模型组不进行干预治疗,针刺组于术后第 2 周给予电针刺激“肾俞” “足三里”和“悬钟” ,每日 1 次,连续治疗 8 周。 通过酶联免疫法( ELISA) 检测血清碱性磷酸酶(ALP) 和骨钙素(OC)含量;通过 Micro-CT 检测股骨骨密度(BMD)和骨形态学参数。 结果 干预 8 周后,与正常组和假手术组相比,模型组和针刺组 ALP 和 OC 升高,BMD、骨体积分数( BV / TV) 和骨小梁厚度( Tb. Th) 减小,骨小梁间隙( Tb.Sp)增大(P<0. 05) ,表明骨质疏松造模成功。 与模型组相比,针刺组 ALP 和 OC 降低,BMD、BV / TV 和 Tb. Th 增大,Tb. Sp 减小(P<0. 05) ,表明针刺能够提高骨密度,改善骨微结构。 结论 针刺具有预防青年期骨质疏松症的作用,其机制可能与针刺促进骨的形成和修复,提高骨密度,增强骨小梁的结构强度相关。

关键词: 针刺, 骨质疏松症, 青年期大鼠, micro-CT