Abstract: Objective To summarize the pre-operative preparation and post-operative nursing experience of neurogenic bladder after complete spinal cord transection in rats. Method Thirty-five female SD rats were purchased, and the rat model of detrusor non-reflex neurogenic bladder was established by transection of S3-S4 spinal cord. The nursing measures were taken before and after operation. It mainly involves the routine nursing before and after operation, the prevention and treatment of post-operative urethral infection and the management of feeding and so on. Result The spinal cord was transected at the S3-S4 segment in all rats, and the post-operative survival rate was 82%. Conclusion Although mature surgical method and skilled operation are important for the model making, careful care and perfect preparation are the key links to ensure the life safety and rapid recovery of animals after operation.

Key words: spinal cord transection, neurogenic bladder, rat model, nursing

摘要： 目的 概述脊髓完全横断后神经源性膀胱大鼠模型术前准备中需要注意的事项及术后护理心得。 方法 购买雌性 SD 大鼠 35 只,横断 S3-S4 脊髓节段,建立逼尿肌无反射型神经源性膀胱大鼠模型,在术前、术后积极实施相关护理措施。 重点涉及手术前后的常规护理、术后尿道感染等并发症的防治及饲养管理等方面的内容。 结果 所有大鼠均在 S3-S4 脊髓节段横断脊髓,术后成活率为 82%(包含 1 只膀胱破裂和 2 只互相撕咬,3 只大鼠死于脓尿后败血症,术后 48 h 成活率为 100%) 。 结论 成熟的手术方法和熟练的手术操作对模型制作固然重要,精心的护理和完善的准备却是保障动物生命安全以及术后迅速恢复的关键环节。

关键词: 脊髓横断, 神经源性膀胱, 大鼠模型, 护理