关键词: 小鼠, 卵母细胞, OPS, SSV

Abstract: Objective To search the new way of freezing preservation of KM mouse's immature oocytes.Methods The immature oocytes from KM mice of 5～6 weeks old were equalized for 5 minutes in 10% EG,or a mixture of 10% EG and 10% DMSO,next were equalized for 30s in different freezing liquids,and then were freezingly preserved by OPS(open pulled straw) and SSV(solid-surface vitrification).Results In mouse's immature oocytes vitrified by OPS,over 84.4% oocytes preserved in EFS were morphologically normal and the mature rate was less than 16.7% after thawing;the 90.5% oocytes preserved in EDFS30 were morphologically normal and the mature rate was 22.5% after thawing;91.9% oocytes preserved in EDFS40,were morphologically normal and the mature rate was 40.5% after thawing,with a very significant difference as compared with the control group(P<0.01).In mouse's immature oocytes vitrified by SSV,86.0% oocytes preserved in EFS were morphologically normal with a mature rate of 46.5% after thawing;92.3% oocytes preserved in EDFS30 were morphologically normal with a mature rate of 57.2% after thawing;90.1% oocytes preserved in EDFS40,were morphologically normal and the mature rate was 48.4% after thawing,with a very significant difference as compared with the control group(P<0.01).In mouse's immature oocytes vitrified by OPS and SSV,morphologically normal rate of the oocytes was respectively 91.6% and 91.4%,the differences were significant as compared with the control group(P<0.01);mature rate of the oocytes was respectively 42.9% and 59.1%,with significant difference as compared with the control group(P<0.01).Conclusion The effect of combination of multiple cryoprotective agents is better than that of single cryoprotective agent,and the effect of EDFS freezing liquid,particularly EDFS30,is better than that of EFS in cryopreservation of mouse oocytes.SSV method is superior in cryopreservation of mouse oocytes to OPS method.

Key words: Mouse, Oocyte, OPS, SSV