关键词: HCG, IVF, 2-细胞胚胎, 受精率, 取卵时间

Abstract: Collecting Oocyte time on IVF rate after HCG injection. Method 3-4 week-old wild-type C57BL / 6J females with 10-13 g were treated with PMSG and HCG. We divided the egg retrieval time of female mice after superarrangement into six periods, after 13, 14, 15, 16, 17 and 18 h for in vitro fertilization. Sperm from the epididymis of 3-month-old male mice were recovered in HTF fluid for 1 h and then used for in vitro fertilization. The experiment was divided into 3 groups, and 12 females were used for overarrangement, and 258,199 and 243 embryos were obtained. In the experiment, 98,87 and 95 births were obtained; the birth rates were 37. 98%, 43. 72% and 39. 09% respectively. Result The total number of eggs and the development rate of 2 cells were the highest at 15 h after superovulation, which showed that the mouse oocytes matured after 14 h, fully mature at 15 to 16 h, and began to degenerate after 17 h of ovulation. Conclusion Comparison of the highest fertilization rate in the 3 groups: the oocyte mass fertilization rate after 15 h in the first group was 79. 17%, the oocyte mass fertilization rate in the second group after 15 h was 75. 68%, and the oocyte mass fertilization rate after
16 h in the third group was 76. 92%.

Key words: HCG, IVF, 2-cell embryo, fertilization rate, collecting Oocyte time