关键词: 钙磷脂结合蛋白Ⅶ, 小鼠模型, 牙齿发育, 动态表达, 牙本质, 分布情况

Abstract: Objective To explore the dynamic expression level of calcium-phospholipid-binding protein Ⅶ (Cpne7) in different stages of mouse tooth development model, and determine its dynamic expression level and distribution during dentigenesis. Method The expression of Cpne7 in the development of the first mandibular molar of C57Bl / 6 mice was studied by immunohistochemistry, in situ hybridization, real-time PCR and Western blot. At the same time, HAT-7 and hDPCs were cultured and analyzed by real-time PCR and Western blot to determine the level of Cpne7 mRNA and protein expression during cell differentiation. Result Cpne7 participates in the early differentiation of ameloblasts and odontoblasts. During initiation ( bud stage) , Cpne7 protein was expressed in dental epithelial cells, but not in dental mesenchyme. At E18 ( bell stage) , Cpne7 protein and mRNA were mainly expressed in the ectodermal mesenchymal cells of the dental papilla. At P7 ( crown formation stage ) , Cpne7 was expressed in differentiated odontoblasts, but weak expression was detected in mature ameloblasts. During dentin generation, Cpne7 was obviously expressed in differentiated dentin cells and odontoblast, but not in fully differentiated dentin cells. In addition, Cpne7 was expressed in the HERS and in the odontoblasts at the time of root dentin formation. Conclusion Cpne7 is not only involved in odontogenic differentiation, but also in the process of odontogenesis and dentin formation of root dentin and crown dentin. Cpne7 shows dynamic spatiotemporal expression during tooth development, and plays an important role in the whole tooth development stage.

Key words: calcium phospholipid-binding protein Ⅶ, murine model, tooth development, dynamic expression, dentin, distribution