关键词: 人参三醇皂苷, 缺血再灌注损伤, 细胞凋亡, 炎症因子

Abstract: Objective To investigate the protective mechanism of ginseng triolsaponin ( PTS) preconditioning on myocardial ischemia-reperfusion injury ( MIRI) in rats. Method After one week of adaptive feeding, 120Wister rats were randomly divided into a sham operation group ( normal saline 2 mL/kg) , a model group ( normal saline 2 mL/kg) , a PTS low dose group ( 25 mg/kg) , and a PTS medium dose group. (45 mg/kg) , PTS high dose group (90 mg/kg) , positive drug group ( shengmai injection 450 mg/kg) .The drug was administered intraperitoneally for 7 consecutive days. MIRI modeling was performed 1 hour after the last administration , and the materials were measured after 24 hours of reperfusion. Monitoring ST segment changes of rat electrocardiogram ； detecting serum creatine kinase isoenzyme ( CK-MB) , interleukin-6 (IL-6) and tumor necrosis factor-— ( TNF-—) levels; detecting myocardial tissue by PCR Bcl-2 and Bax mRNA expression levels ; myocardial histopathology and ultrastructural changes of cardiomyocytes were observed by light and electron microscopy. Result Compared with the model group , the PTS medium dose group and the high dose group can significantly reduce the STsegmentand serum CK- MB, IL-6, TNF-— levels in the MIRI model rats, improve the damage of myocardial structure, and increase the anti-apoptotic protein Bcl-2 expression inhibits the expression of the proapoptotic protein Bax. Conclusion PTS has protective effects on MIRI rat myocardium , and its mechanism maybe achieved by inhibiting the expression of inflammatory factors and regulating keyenzymes of apoptosis.

Key words: Ginseng triolsaponin, ischemia-reperfusion injury, apoptosis, inflammatory factor