关键词: 小鼠肝炎病毒, 隔离检疫, 哨兵鼠, 检测

Abstract: Objective To explore the study design of pathogenic microorganism infection in experimental animals , this study used two classical strategies to detect the change of virus load and serum specific antibody in faeces of MHE- infected mice. Method C57BL/6 and BALB/c mice ( n = 20 ) were divided into 4 cages and fed with litter from MHE- infected animals for 28 days. After which each animal was kept in an isolated cage with aseptic litter, the fecal and serum were collected per week. Samples were tested by Taqman real-time PCR and ELISA , and the data were analyzed statistically. Result For BALB/c mice, MHE was detected as early as d21 post infection (positive rate of 20%) and reached to the highest with 80% infection rate at d 28 , then decreased to 50% at d42, and can not be detected atd56 when using qPCR method .While positive result were detected atd42 with infection rate of 50% when using serum ELISA method. For C57BL/6 mice, MHE was detected at d28 and d42 post infection in only 20% of mice when using qPCR method , and no positive antibodies were detected during experimental period when using serum ELISA method. Conclusion For mouse hepatitis virus detection, BALB/c mice were recommended as sentinel mice in isolation quarantine and routine pathogen detection , and qPCR method was recommended for early isolation quarantinewith the advantage of earlier and higher detection rate.

Key words: Mouse hepatitis virus, quarantine, sentinel mouse, detection