关键词: 鞘内给药, 大鼠, 枕寰膜, 腰椎椎间孔

Abstract: Objective Based on experiments, an intrathecal administration method which is stable , reliable, easy, continuous and less damage was presented. Method Rats were randomly underwent catheter implantation either through atlanto-occipital membrane or foramen intervertebrale , the out erend of the catheters were either linked toa Alzet Osmotic Pump or just fixed to skin. According to the different treatment pattern , the animals were divided into 6 groups: 4 operation groups (atlanto-occipital catheterization group , atlanto-occipital catheterization with Osmotic Pump group , foramen intervertebrale catheterization group , foramen intervertebrale catheterization with Osmotic Pump group ) and 2 sham groups ( atlanto-occipital catheterization sham group , foramen intervertebrale catheterization sham group ). Death , paralysis , and sighs of developing motordys function and ill health were abserved after surgery. Rotarod motor function , paw with drawal threshold ( PWT ) and paw withdrawallatency (PWL) were also tested before and after surgery. Result Only half of the rats underwent atlanto-occipital catheterization could beused for further study , the others developed motordysfunction , ill health ( see page of eyes and nose , weight loss ) orweredead. Because of dislodging of the catheter , one-third softhe animals underwent for amen intervertebrale catheterization were excluded from further study , the other two-thirds of the rats were qualified. While all of the rats underwent foramen intervertebrale catheterization connecting to Osmotic Pump could beused for further study. Atlanto-occipital catheterization could resultin hyperalgesia and decrease of rotarod motor function.The duration of running times on rotarod , PWT and PWLwere all significantly lower than that of sham group and before-operation state. Foramen intervertebrale catheterization had no significante fecton PWT , PWL and the running time son rotarod. Conclusion Iintervertebrale catheterization combining with Osmotic Pump was a stable , reliable intrathecal adminidtration method with less damage.

Key words: Intrathecal administration, rat, Atlanto-occipital membrane, Foramen intervertebrale