关键词: 行为箱形状, 小鼠, 学习记忆, 新物体识别实验

Abstract: Object To investigate the effects of the result of novel object recognition tests in different experimental conditions with ICR mice. Method Divided 40 ICR mice(half male and female)into 4 groups randomly, the square female group (the female mice group working in the square box), the square male group, the round female group and the round male group. According to the same experimental process of novel object recognition, the exploration time of mice in each group was measured. Result There was no significant differences between the square female group and the square male group, the round female group and the round male group in the total exploration time and the discrimination ratio of novel object (P>0.05). Compared the square group(the square female group+the square male group) with the round group (the round female group + the round male group), there was no significantly differences in the discrimination ratio of novel object (P>0.05), but the total exploration time of the round group was significantly longer than that of square group (P<0.01). Conclusion In the experiment of novel objects, the sex of mice did not affect the evaluation of learning and memory ability of mice, and the choice of round behavior box for experiments could better evaluate the learning and memory ability of mice.

Key words: the shape of the testing environment, mice, learning and memory, novel object recognition test