关键词: 实验用猫, 通体, 实时荧光定量PCR, 应用

Abstract: Objective To establish an effective and specific real-time PCR assay for Bartonella detection, and use the assay in experimental cat. Method According Bartonella RIBC gene fragments, a pair of specific primers and TaqMan probe were designed and synthesized. Bartonella DNA standards were prepared using some molecular biological method . Then the linearity, sensitivity, specificity and stability of the method were measured. At last, the method was used for testing blood samples from 142 cats. Result We established successfully real-time PCR method for Bartonella detection. The linear range was 1.0×101～1.0×109 copies/μL, the lowest sensitivity was 10 copies/μL, the coefficient of variation (CV) was 0.998. There was no false positive detection from other bacterial strains. 6 positive reactions were detected in the blood samples of 142 cats. Conclusion The method is able to be used to detect and quantify the Bartonella in experimental cats.

Key words: experimental cat, Bartonella, real-time RT-PCR, application