关键词: <, p>, 颅脑损伤, 颅脑损伤仪, 动物模型<, /p>

Abstract: Abstract: Objective To study on preparation of TBI rats model by eCCI，and provide a model preparation method for the TBI experimental study． Method SD rats，which were randomly divided into blank control group，model group 1，model group 2，model group 3 and sham-operation group，were produced as the animal model of brain injury in different degree by eCCI from selected different fighting parameters． The speed was 3 m / s and the depth was 3 mm in model group 1． The speed was 4 m / s and the depth was 3 mm in model group 2． The speed was 5 m / s and the depth was 3 mm in model group 3． Evaluated the model quality through the following: To detect the contents of Bax and Bcl-2 of brain tissue by immunohistochemical method ． Ｒesult The Bax、Bcl-2 in the brain tissue of cerebral cortex of neurons showed weak positive reaction in control group and sham operation group． The Bax、Bcl-2 of damaged brain in model group showed positive reaction and the expression was markedly increased with the increase of hit ( P ＜ 0. 01) ． Conclusion By eCCI TBI rat model can be prepared with regulation of hit speed and depth． The damage of model was related to the hit speed． The method of TBI model preparation has the advantages of easy operation，good control ability，repeatability，low mortality rate，and can according to the need be used to make various animal model for the study．

Key words: <p>brain injury, eCCI, rat model </p>