关键词: <, p>, 鼠脑心肌炎病毒, EMV, TaqMan 探针, 荧光定量 ＲT-PCＲ<, /p>

Abstract: Abstract: Objective To develop a real-time ＲT-PCＲ assay ( rＲT-PCＲ) for efficient detection of Murine Encephalomyocarditis virus ( EMV) ． Method According to the genomic sequences of EMV strain PEC9 ( DQ288856. 1) download from NCBI and to find the conserved sequences． One pair of the specific primers and one TaqMan probe were designed． Then reaction parameters were optimized to develop a real-time ＲT-PCＲ assay ( rＲTPCＲ) ． Ｒesult The method showed a good linear relationship of standard curve with a r 2 value of 0. 99． The sensitivity of the real-time PCＲ was less than 1 copies /μL． 1 000 times higher than ordinary PCＲ method ，and its specificity is strong，common mice strains had no specific amplification，between batch and batch of variation coefficient is less than 2% ． 120 mice feces samples epidemic materials provided by the Shanghai institute of experimental animals were detected by the real-time PCＲ． All the 120 mice samples were negative． Conclusion The real-time ＲT-PCＲmethod is good in specificity and sensitivity，which can make a powerful technical support for EMV epidemiological investigation and detection．

Key words: <p>Murine Encephalomyocarditis virus, EMV, TaqMan, real time PCＲ</p>