关键词: <p>长爪沙鼠, 2 型糖尿病, Mylpf, 实时定量荧光 PCＲ, Western blot</p>

Abstract: Abstract: Objective To analysis of Mylpf expression levels in six tissues of diabetic Mongolian gerbils and to explore the molecular mechanism of diabetes in Mongolian gerbils． Method We selected a diabetic group and a control group，each group has 6 Mongolian gerbils． Mylpf mＲNA and protein expression level in skeletal muscle， liver，adipose，kidney，heart and brain were separately detected using Ｒeal-time PCＲ and Western blotting．Ｒesult The Ｒeal-time PCＲ showed that compared with control animal，mＲNA expression of Mylpfin skeletal muscle，heart and brain was increased in diabetic group． The relative expression level in diabetic group exhibited decreased compared with control in adipose and liver． And there were no differences between two groups in the kidney． The Western blotting result display that protein of Mylpf in skeletal muscle was significantly increased in diabetes group． The expression of Mylpf in diabetic Mongolian gerbils heart and adipose was lower than control group． Mylpf protein was almost undetectable in liver and brain tissue． And Varied expression was observed in kidney，but there is no difference． Conclusion There is a correlation between Mylpf and the occurrence of diabetes in Mongolian gerbils and theresult show that the effect of Mylpf mainly occurred in skeletal muscle and heart tissue of diabetic gerbils．

Key words: <p>Mongolian gerbils, Type 2 diabetes, Mylpf, Ｒeal time PCＲ, Western blotting</p>