关键词: <, p>, 癫痫清颗粒, 戊四唑, AIF, BaX, Bcl-X/ S + L<, /p>

Abstract: Abstract: Objective This study was to investigate the effects of DXQ on pentylenetetrazole-induced hippocampal pyramidal cells apoptosis and cerebrum damage，then to explore its possible mechanisms． Method Male Wistar rats were randomly divided into 7 groups: normal control ( NC) ，pentylenetetrazole ( PTZ) ，Dian-Xian-Qing granule( DHQ) ( 4. 78，9. 46，18. 92 g / kg·d － 1 ) ，Dian-Xian-Ning Tablets( DXN，5. 05 g / kg·d － 1 ) and Phenytoin Sodium Tablets ( PST，54 mg / kg·d － 1 ) positive control groups，a sub-convulsive dose of PTZ 40 mg / kg in the volume of 1 mL / kg( ip) was administered to rats on every second day for 30 days resulting in a permanent change of generalized tonic-clonic convulsions． The convulsive behaviors were evaluated according to Ｒacine's score，the number of surviving hippocampal pyramidal cells was assessed by HE staining． The expression of AIF ( apoptosis inducing factor) ，BaX and Bcl-x / s + L were analyzed by Western blot immunohistochemical ( IHC) staining．Ｒesult Ｒats suffered PTZ showed higher Ｒacine's score，worse cell damage and apoptosis，which were all attenuated by DHQ ( 4. 78，9. 46，18. 92 g / kg·d － 1 ) ． Moreover，DHQ reversed the expression of AIF，BaX and Bcl-x / s + L induced by tonic-clonic convulsion． Conclusion These result suggest that DHQ has neuroprotective effects，which is related to the level of AIF，BaX and Bcl-x / s + L restraining cells apoptosis．

Key words: <p>Dian Xian Qing granule, pentylenetetrazole, AIF, BaX, Bcl x / s + L</p>