关键词: 成蛙, 灌胃, 毒性, 给药

Abstract: Abstract:To explore a new way to administrate drugs to the adult frog． Adult tiger frogs ( Ｒana rugulosa) at 90- day-old were fed with a mixture of pesticide (Bt suspension fluid) and forage． The frogs were randomly divided into blank control and five dosage groups (1 000，2 150，4640，10 000，21 500 mg / kg) ． Four repeats were conducted for each group and 5 frogs were administrated in each repeat． The median lethal dose ( LD50 ) and body weight gain were recorded to evaluate the feasibility of oral gavage in adult frogs in acute toxicity test． The results showed the oral gavage can make the frogs get nutrition support and gain weight． Moreover，there was a strong positive relationship between dose and response，and the LD50 value was 6 691 mg / kg． Therefore，the adult frogs can be administrated with various drugs by oral gavage，which will provide a new workable method for frog toxicity test．

Key words: frog, oral gavage, toxicity drug administration