关键词: 进展性动脉粥样硬化, 脂质核心, 兔模型, 球囊损伤法

Abstract: Abstract:Objective To develop a rapid and efficient method to establish the advanced atherosclerosis model． Method Fifteen New Zealand white rabbits were randomized into common carotid arteries (CCAs) balloon injury group ( n = 8 ) and sham-operated group ( n = 7 )，and fed with high-fat diets for fourteen weeks． Pathological characteristics of CCAs and other vascular segments，thoracic aortae，aortic roots and abdominal aortae，were observed． To assess the size of plaques，the percentage of intima proliferation area in internal elastic lamina ( IEL) area，average intima thickness in the radius of IEL and maximal intima thickness in the radius of IEL were calculated． Severity of lesions was evaluated by determining the histological classification recommended by American Heart Association． The serum lipid profiles of animals before and after experiment are measured． Ｒesult The size of plaques in thoracic aortae，aortic roots and abdominal aortae was decreased after fourteen-week highfat diet feeding． Although the biggest plaque and fastest lesion progression rate were observed in thoracic aortae ， the intermediate lesions were still the main manner characterized by intimal small extracellular lipid pools，rarely developing advanced lesions characterized by lipid core formation． The size of plaques in CCAs injured by balloon was bigger significantly than that of thoracic arotae ( P ＜ 0. 01 )，which was characterized by advanced atherosclerotic lesions． Conclusion Combined with high-fat diet feeding，advanced atherosclerosis model characterized by lipid core can be established rapidly and efficiently by balloon injury．

Key words: advanced atherosclerosis, lipid core, rabbit model, balloon injury