关键词: 树鼩, 奇异变形杆菌, 分离鉴定, 药敏试验

Abstract: Abstract:To identify the pathogen from diarrheic tree shrews which may provide an useful reference for preventing and treatment diseases in tree shrews ( Tupaia belangeri Chinensis) ． 6 fresh feces were collected，and identified the dominant and suspicious bacteria by cultural characteristics，colony morphology，staining，biochemical testing and molecular biology． Antibiotic sensitivity of isolated strains were detected with 8 kinds of antibiotics． Most colonies were white-yellow with smooth and damp surface on nutrient agar plates，round with dark cores． Some of them spread black ripples and smelly odour． Bacterial character were brevibacterium with blunt，without spore and capsule，size were (0. 4 ～ 0. 6 μm) × (1. 0 ～ 3. 0 μm)，gram negative and non acid fast bacteria． The isolated strain produced hydrogen sulfide． The reactions of ornithine ，urea etc were positive． Genetic similarity were 99% with the Proteus mirabilis from GenBank． All results showed the isolated was a strain of Proteus mirabilis． The isolated strains were sensitive to all tested antibiotics． The Proteus mirabilis isolated from diarrheic tree shrews is the pathogenic agent leading to diarrhea and death in lactation tree shrews and infant． It was be controlled by improving management and treatment．

Key words: tree shrews, Proteus mirabilis, isolation and identification, antibiotic sensitivity test