关键词: 小鼠肝炎病毒, RT-PCR, Real-time RT-PCR

Abstract: Abstract: Objective To establish the methods of nucleic acid detection for mouse hepatitis virus ( MHV) so as to meet the need of rapid quarantine for rodent animals in the port of entry． Method Using primer and TaqMan fluorescent probe software，the primers and probe were designed to target conserved gene of MHV． And methods of RT-PCR and Real-time RT-PCR using TaqMan probe were established to detect MHV from the clinical samples of rodent animals． Result The two established methods for MHV detection were proved to be specific with high sensitivity and efficiency． Using these two methods to detect clinical samples collected from MHV positive mouse， the results show RT-PCR and Real-time RT-PCR methods have high stability and reliablity． Conclusion The methods of RT-PCR and real-time RT-PCR to detect MHV were established，which are suitable to detect easily available samples such as feces and urine．

Key words: Mouse hepatitis virus, RT-PCR, Real-time RT-PCR