关键词: p53, 基因敲除小鼠, PCR

Abstract: Abstract: Objective To breed and identify p53 gene knockout mice，Heterozygote mice were bred and reproduced． Methods Genome DNA extracted from the mice’s tails were subjected to PCR test for genotype identification． Results Heterozygous were used to acquire baby mice for Protection species． Conclusion The breeding and reproducing were successful and Homozygous genotype mice were acquired． Appropriate methods of feeding，breeding and identifying are important for obtaining gene knockout mice and protecting species．

Key words: p53, gene knockout mice, PCR