关键词: 屏障级环境, 实验动物, 消毒验证

Abstract: Objective To explore the disinfection effects of 6 disinfectants, namely 3% hydrogen peroxide, 75% ethanol, 84 disinfectant, brand 1 disinfectant, brand 2 disinfectant and potassium bisulfate, in animal houses in barrier environment, so as to provide reference for actual disinfection work.  Method The 6 disinfectants were diluted accordingly according to the product instructions, and the cage boxes of SPF mice were prepared, and the dirty bedding was fully rubbed to make the microorganisms fully contact with the gloves, and the experimental group used sterile gloves to rub the dirty bedding and then disinfect it with different method, Then sampled the surface of the gloves, the sampling positions were all palms and backs, the sampling area was 5 cm×5 cm, the sample solution was smeared on the agar petri dish for bacterial culture, Then positive control was directly sampled and cultured after rubbing the dirty bedding. The negative control samples were smeared on the surface of sterile medical gloves, All experimental groups and the positive and negative control groups were treated with 3 sterile agar medium for 48 h at 37 ℃ to count the growth colonies, and the disinfection effects of different treatment method were compared. Result No colony growth was observed after 75% ethanol disinfection. Brand 2 disinfectant solution was diluted and soaked in 1 ∶ 200 and 1 ∶ 400 for disinfection, and no colony growth was observed in the sampling culture. The average number of colonies grown after 3% hydrogen peroxide disinfection was ( 0. 33 ± 0. 47) cfu / dish. The average number of colonies after disinfection of brand 2 disinfectant solution (1 ∶ 128 dilution) was (53. 3±3. 9) cfu / dish, the average number of colonies after spray disinfection of brand 1 disinfectant solution ( 1 ∶ 100 dilution ) was ( 52. 3± 4. 5 ) cfu / dish, potassium persulfate ( 1 ∶ 100 dilution) was ( 68. 3 ± 5. 3) cfu / dish, 84 disinfectant solution ( 1 ∶ 50 dilution) average colonies were (62. 0±2. 4) cfu / dish, and potassium persulfate (1 ∶ 200 dilution) and other disinfectant spray disinfection method, the average number of colonies was (145. 7±5. 7) cfu / dish, and the average number of colonies after spraying of brand 2 disinfectant (1 ∶ 200 dilution) was (178. 3 ±2. 1) cfu / dish. The number of colonies grew in the positive control > 500 cfu / dish, and the colonies grew in the negative control. Conclusion The order of disinfection effect is 75% ethanol, 3% hydrogen peroxide spray and brand 2 disinfectant soaking, high concentration ( brand 1 disinfectant, potassium persulfate and 84 disinfectant) spray disinfection, and low concentration potassium persulfate and low concentration brand 2 disinfectant spray. The validation result can provide a reference for the use of disinfectants in animal rearing processes. 

Key words: barrier-level environment, experimental animal, disinfection verification