高原缺氧寒冷应激环境功能性肠病大鼠模型胃肠组织病理学变化

doi:10.3969/j.issn.1006-6179.2024.05.009

实验动物科学 ›› 2024, Vol. 41 ›› Issue (5): 48-54.DOI: 10.3969/j.issn.1006-6179.2024.05.009

高原缺氧寒冷应激环境功能性肠病大鼠模型胃肠组织病理学变化

( 1. 新疆农业大学动物医学学院, 乌鲁木齐 830052) ( 2. 新疆军区总医院新疆特殊环境医学重点实验室, 乌鲁木齐 830000)

( 3. 军队高原高寒医学保障重点实验室, 乌鲁木齐 830000)

收稿日期:2023-12-31 出版日期:2024-10-28 发布日期:2024-10-28
通讯作者: 许琴( 1974—) ,女,高级实验师,博士,研究方向为人类疾病动物模型制作,特殊环境医学病理生理学,E-mail:xuqin740831@ 163. com。赵红琼( 1974—) ,女,教授,研究方向为基础兽医学,生物活性物质结构与功能,E-mail:zhaohongqiong@ sina. com。
作者简介:买力达·马和木提( 1998—) ,女,硕士研究生,研究方向为基础兽医学,E-mail:1439044436@ qq. com。
基金资助:
全军实验动物专项课题( SYDW[ 2018] 10 号)

Changes of Gastrointestinal Histopathology in Rat of Functional Bowel Disorder induced by Hypoxic Cold Stress at Simulated High Altitude Environment

( 1. Veterinary Medicine College of Xinjiang Agricultural University, Urumqi 830052, China)

(2. General Hospital of Xinjiang Military Command, Key Laboratory of Special Environmental Medicines of Xinjiang, Urumqi 830000,China)

( 3. Key Laboratory of High Altitude and Frigid Zone Medical Support, PLA, Urumqi 830000, China)

Received:2023-12-31 Online:2024-10-28 Published:2024-10-28

摘要/Abstract

摘要：

目的探讨高原缺氧寒冷应激环境功能性肠病( FBD)大鼠胃肠组织病理学变化特点并探究微生态制剂干预

对其胃肠组织的保护效果。方法建立高原缺氧寒冷应激环境 FBD 大鼠模型,采集平原对照组( PY) 、平原模型组

( PM) 、高原对照组(GD)和高原模型组(GM)及 4 种微生态制剂干预组大鼠的胃、回肠和结肠组织进行 HE 染色,

光镜下观察各组织结构变化并进行病理学损伤评分。结果与 PY 组比较,PM 组、GD 组和 GM 组大鼠肠黏膜均可

见部分黏膜上皮细胞脱落坏死,固有层裸露,黏膜下层及黏膜下间质、黏膜肌层毛细血管扩张充血,少量炎性细胞

浸润。与 GM 组相比,微生态制剂干预组大鼠的病理损伤明显减轻,其中 GS、FMT 组干预效果优于 DR 和 DRP 组。

结论高原缺氧寒冷应激环境功能性肠病大鼠胃肠组织发生明显的病理学改变,微生态制剂干预对胃肠组织有一

定的保护作用。

关键词:

缺氧寒冷应激环境, 功能性肠病模型, 病理损伤, 微生态制剂

Abstract:

Objective To investigate the characteristics of gastrointestinal histopathological changes in

rats with Functional Bowel Disorder ( FBD) exposed to hypoxia and cold stress at high altitude and the

protective effect of microecological agents on the gastrointestinal tissue. Method The rat model of FBD

under hypoxic cold stress at high altitude was established, and the gastric, ileum and colon tissues of the

rats in the plain control group ( PY) , plain model group ( PM) , plateau control group ( GD) , plateau

model group (GM) and four kinds of microecological preparation intervention groups were collected for

HE staining. The structural changes of the tissues were observed under light microscope and pathological

damage scores were performed. Result Compared with PY group, part of mucosal epithelial cells were

exfoliated and necrotic, lamina propria was exposed, submucosal and submucosal interstitium and

mucosal myoma were dilated and congested, and a small amount of inflammatory cells were infiltrated in

the intestinal mucosa of PM, GD and GM groups. Compared with GM group, the pathological damage of

rats in the microecological preparation group was significantly reduced, and the intervention effect of GS

and FMT groups was better than that of DR And DRP groups. Conclusion There are obvious

pathological changes in the gastrointestinal tissues of rats with Functional Bowel Disorder under hypoxic

and cold stress at high altitude, and the intervention of microecological preparations has a certain

protective effect on the gastrointestinal tissues.

Key words:

hypoxic cold stress environment, functional bowel disorder model, pathological injury;

microecological preparation

中图分类号:

Q95-3

买力达·马和木提, 雷艳, 李建瑛, 廖西江, 宋来阳, 赵红琼, 许琴.

高原缺氧寒冷应激环境功能性肠病大鼠模型胃肠组织病理学变化

[J]. 实验动物科学, 2024, 41(5): 48-54.

MAILIDA Mahemuti, LEI Yan , LI Jianying , LIAO Xijiang , SONG Laiyang, ZHAO Hongqiong, XU Qin .

Changes of Gastrointestinal Histopathology in Rat of Functional Bowel Disorder induced by Hypoxic Cold Stress at Simulated High Altitude Environment

[J]. Laboratory Animal Science, 2024, 41(5): 48-54.

高原缺氧寒冷应激环境功能性肠病大鼠模型胃肠组织病理学变化

Changes of Gastrointestinal Histopathology in Rat of Functional Bowel Disorder induced by Hypoxic Cold Stress at Simulated High Altitude Environment

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