关键词: 石蜡切片, HE 染色, 小鼠肝, 小鼠肠道, 脱水, 浸蜡

Abstract: Objective To compare the effects of two dehydration procedures and five wax immersion timeson the quality of paraffin sections of mouse liver and intestinal tissues, with a view to optimizing thepreparation procedures of paraffin sections. Method The liver and intestinal tissues of BALB / c micewere prepared in two different dehydration procedures ( Procedure I and Procedure II) and five differentwax immersion procedures ( total immersion time of 90, 120, 150, 180 and 210 min) to compare theresult of tissue sectioning and HE staining. Result The paraffin sections produced by dehydrationprocedure 1 were easily fragmented, with obvious wrinkles and cavities in the center of the tissue; theparaffin sections produced by dehydration procedure 2 were intact and free of cavities, and the structureof the paraffin sections was observed to be intact and clear under the microscope after HE staining.Compared with the other four wax immersion procedures, the paraffin sections produced with a totalimmersion time of 150 min were the most complete and clear after staining. Conclusion The quality ofthe paraffin sections produced by using the dehydration procedure II and the total time of wax immersionof 150 min was improved and easy to observe under the microscope, which is recommended as theoptimized procedure for paraffin sections of mouse liver and intestinal tissues.

Key words: paraffin section, HE taining, mice liver, mice intestinal, dehydration, wax immersion