关键词: Nrf2, 肛瘘, 血管新生, 黛玉膏, HO-1 

Abstract: Objective To observe the effect of Daiyu ointment on angiogenesis and Nrf2 / HO-1 signaling pathway in rats with anal fistula. Method 20 SPF-grade SD rats were constructed a post-operative wound model of fistula by comprehensive method, after successful modeling, they were randomly divided into model group and Daiyu paste group, with 10 rats in each group. Rats in the Daiyu paste group applied the wound with drugs, and the model group scrubbed with 0. 9% sodium chloride solution. In addition, 10 rats with only wounds were selected as the control group, and scrubbed with 0. 9% sodium chloride solution, and the rats in each group were treated twice a day, all of which were continuously intervened for 14 days. then the wound healing and local blood flow was observed. Then the rats were sacrificed. ELISA was used to detect the levels of vascular endothelial growth factor ( VEGF) , VEGF receptor (VEGFR) , epidermal growth factor (EGF) , platelet derived growth factor ( PDGF) , interleukin ( IL) - 1β, IL-6, IL-10, tumor necrosis factor α ( TNF-α) in wound tissues of anal fistula; Western blot was used to detect the protein levels of Nrf2 and HO-1 in the wound tissues. Result Compared with the control group, the wound healing rate and blood flow of the model group significantly decreased ( P < 0. 05) . Compared with the model group, the wound healing rate and blood flow of rats in the Daiyu ointment group significantly increased (P<0. 05) ; Compared with the control group, CD31 positive area significantly decreased (P<0. 05) , while significantly increased in Daiyu ointment group compared with model group; The levels of IL-1β, IL-6, IL-8 and TNF-α in granulation tissue were significantly higher in the model group than in the control group ( P < 0. 05) , and the Daiyu cream group was significantly lower than that in the model group ( P< 0. 05) ; wound tissue VEGF, VEGFR, EGF and PDGF in the model group were significantly higher than those in the control group (P<0. 05) , and the Daiyu ointment group was significantly higher than the model group (P<0. 05) ; the levels of Nrf2 and HO-1 protein in the model group were significantly higher than those in the control group significantly higher (P<0. 05) , and the Daiyu ointment group was significantly higher than the model group ( P< 0. 05) . Conclusion Daiyu ointment can promote the healing of wounds and angiogenesis in anal fistula model rats. The mechanism may be related to the activation of Nrf2 / HO-1 pathway. 

Key words: Nrf2, anal fistula, angiogenesis, Daiyu ointment, HO-1