关键词: 环磷酰胺, 兔, 发育毒性, 胚胎-胎仔

Abstract: Objective To explore the method of establishing rabbit teratogenicity experimental model by oral gavage of cyclophosphamide in the developmental toxicity test of embryo-fetal rabbits, and to provide laboratory background data for the experimental study of teratogenicity. Method Sixty-six healthy pregnant ordinary New Zealand rabbits were selected and divided into lysozyme control group ( 0. 5% sodium carboxymethyl cellulose) and cyclophosphamide poisoning group (20 mg / kg) by random number method according to the weight of objects. After successful mating, pregnant rabbits were given 2 mL / kg intragastrically infected on GD6 - GD18. During the poisoning period, the clinical manifestations were observed, body weight, successful conception rate, luteal number, number of live births, number of stillbirths and number of absorbed fetuses of pregnant rabbits were counted, and body weight, length, appearance deformity, visceral deformity and skeletal deformity of the fetuses were recorded. Result There was no significant difference in body weight between cyclophosphamide group and lysozyme control group(P > 0. 05 ) . The number of luteal body, number of live births and number of implantation of pregnant rabbits in cyclophosphamide group were decreased compared with lysozyme control group, and the differences were statistically significant(P<0. 05) . Fetal weight, body length and placental weight in cyclophosphamide group were all decreased compared with lysozyme control group, and the differences were statistically significant ( P < 0. 05 ) . The appearance, visceral and skeletal abnormalities were significantly increased in the cyclophosphamide group compared with the lysozyme control group, and the difference was statistically significant(P<0. 01, P<0. 05) . Conclusion Cyclophosphamide was poisoned by oral gavage at the dose of 20 mg / kg has a short administration period, a high rate of fetal deformities, a wide variety of deformities, and no maternal toxicity. Therefore, a better positive model of embryo-fetal developmental toxicity can be established.

Key words: cyclophosphamide, rabbit, development toxicity;embryo-fetal