关键词: 关键词:阿尔茨海默病, 大鼠, 动物模型, 三氯化铝, D-半乳糖

Abstract: Abstract: Objective To establish the Alzheimer’ s disease ( AD) rats model induced by multi-factors. Method Twenty SD rats were randomly divided into the control group and the model group, with 10 rats in each group. To build the AD models, D-galactose ( 60 mg / kg) was injected intra-abdominally, meanwhile, aluminium chloride (100 mg / kg) was hypodermically injected for 45 days. The learning and memory abilities of model rats were assessed using Morris water maze test. The level of acetyl cholinesteraseinin rat brain tissue was also measured. Result In the Morris water maze test, compared with the control group, the escape latency of the rat in the model group was significantly prolonged ( P<0. 01) , through the time and distance in target area was obviously increased, the number of active explorations through the platform was significantly reduced ( P < 0. 01 ) . The acetyl cholinesterase levels in brain tissue were increased significantly ( P < 0. 01) . Conclusion A complicated AD rat model was successfully established by combined intervention of D-galactose and aluminium chloride.

Key words: Key words:Alzheimer’ s disease, rats, animal mode, aluminium chloride, D-galactose