关键词: 基因工程小鼠, 代乳, 剖宫产手术, 生物净化

Abstract: Objective To obtain SPF healthy mice and improve the purification efficiency of cesarean section. Method The following experiments are involved in this study, such as judging the development of the fetus by palpation to accurately determine the start time of the cesarean section, by optimizing the cesarean section procedure, shortening the operation time to improve the efficiency of cesarean section, improving the milking method of the excised pup rate and doing statistical method to analyze the cesarean section purification data. Result A. By optimizing and improving the surgical procedure, the success rate of uterine section surgery reached ( 97. 9± 8. 25) %; B. when the time interval was less than 24 hours, the average lactation rate was 91. 2%; C. the microbiological grades of the pups purified by cesarean section technology reached the SPF level. Conclusion The perfect cesarean section technique not only improves the microbiological grade of laboratory mice, but also greatly improves the success rate of surgery, and laying the foundation for the biological purification of laboratory mice.

Key words: genetically engineered mice, foster nursing, cesarean section, biological purification