实验动物科学 ›› 2021, Vol. 38 ›› Issue (6): 63-.

• • 上一篇    下一篇

实验动物病理组织切片质量评价体系与回顾性分析 

  

  1. (首都医科大学实验动物部,北京 100069)
  • 出版日期:2021-12-28 发布日期:2022-01-18

Experience and Technique of Making Pathological Tissue Sections of Laboratory Animals 

  1. ( Department of Laboratory Animal Sciences, Capital Medical University, Beijing 100069, China)
  • Online:2021-12-28 Published:2022-01-18

摘要: 目的 分析前期制作的实验动物病理切片质量,为提高切片制作水平提供借鉴及指导方法 随机选取1800 件切片作为研究对象,根据相关质量控制标准评分,统计坏片率并对坏片原因予以分析结果 切片中,优片率为 90. 17% (1 623 / 1 800) ,坏片率为 9. 83% (177 / 1 800) 。 其中,甲级片占比为 31. 50% ,坏片率在可接受范围之内结论 规范取材操作,根据实验动物品种年龄等因素,合理选择固定液和固定时间,严格控制包埋过程,提升切片技术,适时更换试剂试药等有助于改善实验动物病理切片质量,进一步提高甲级片,助力科学研究工作

关键词: 病理切片, 质量, 坏片率, 制作技巧

Abstract: Objective The quality of pathological sections of laboratory animals made in the early stage was analyzed to provide reference and guidance for improving the level of section making. Method A total of 1800 slices were randomly selected as the research objects, the failure rate was calculated according to the relevant quality control standards, and the causes of the failure were analyzed. Result The optimal slice rate was 90. 17% (1 623 / 1 800) , and the bad slice rate was 9. 83% ( 177 / 1 800) . The proportion of grade a film is 31. 50%, which should be further improved. The failure rate is within the acceptable range. Conclusion Standardized sampling operation, reasonable selection of fixed solution and fixed time according to the species and age of laboratory animals, strict control of embedding process, improvement of sectioning technology, timely replacement of reagent and other factors can help improve the quality of pathological sections of laboratory animals and facilitate scientific research.

Key words: pathological section, quality, bad slice rate, production techniques